超声提取优选研究多叶棘豆各药用部位盐酸小檗碱的分布

Study on the Distribution of Berberine Hydrochloride in Various Medicinal Parts of Oxytropis myriophy by Ultrasonic Extraction Op-timization

doi:
摘要:
[目的]比较2种超声提取工艺研究多叶棘豆茎、叶、根、种子中盐酸小檗碱分布的不同.[方法]采用2种超声提取工艺对蒙药多叶棘豆茎、叶、根、种子中盐酸小檗碱进行提取并优选(方法Ⅰ是先将蒙药进行超声提取后放置24 h,方法Ⅱ是将蒙药先室温放置24 h再进行超声提取).在波长426 nm处用分光光度法测定,计算盐酸小檗碱的含量和回收率.[结果]多叶棘豆茎中盐酸小檗碱分布最多,叶、根、种子盐酸小檗碱分布明显减少,而2种提取工艺中方法Ⅱ优于方法Ⅰ,加样回收率在92.58%~110.00%,RSD在0.7823%~0.9749%.[结论]多叶棘豆各药用部位盐酸小檗碱分布不均,茎中分布明显多,2种不同超声提取工艺中方法Ⅱ优于方法Ⅰ.
Abstract:
[Objective] The research aimed to compare the distribution of berberine hydrochloride in the stem, leaf, root and seed of Oxytropis myriophy by two different ultrasonic extraction techniques.[ Method] Two kinds of ultrasonic extraction process were used to extract and optimize the berberine hydrochloride in the stems, leaves, roots and seeds of Oxytropis myriophy(The first technology was to extract the Mongolian medicine first by ultrasonic extraction and then placed 24 h, and the second technology was to put the Mongolian medicine at room temperature for 24 h before ultrasonic extraction.).And calculated the content of berberine hydrochloride and recovery in 426 nm wavelength spectrophotometry.[Result]The content of berberine hydrochloride was the most in the stalk of Oxytropis myriophy, the distribution of berberine hydrochloride in leaves, roots and seeds was obviously reduced,and the second technology of extraction process was better than the first technology , the recovery rate was 92.58%-110.00%, the relative standard deviation (RSD) was 0.7823%-0.9749%.[Conclusion]The distribution of berberine hydrochloride is not equal in the medicinal parts of Oxytropis myriophy, and the distribution in the stems is much more significantly, and the second technology is better than the first in the two different ultrasonic extraction techniques .
作者 海平[1]    苏雅乐其其格[2]
Author: HAI Ping[1]    Suyaleqiqige[2]
作者单位
  1. 内蒙古民族大学化学化工学院,内蒙古通辽,028000
  2. 内蒙古民族大学农学院,内蒙古通辽,028000
刊 名: 安徽农业科学   ISTICPKU
Journal: Journal of Anhui Agricultural Sciences
年,卷(期) 2017, 45(36)
分类号 S567
关键词: 超声提取 知识脉络    优选 知识脉络    多叶棘豆 知识脉络    不同药用部位 知识脉络    盐酸小檗碱 知识脉络   
Keywords: Ultrasonic extraction    Optimization    Oxytropis myriophy    Different medical parts    Berberine hydrochloride   
机标分类号
基金项目 内蒙古民族大学国家级培育项目,内蒙古民族大学天然产物化学及功能分子合成自治区重点实验室开放课题
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