UPLC法测定整参·须根和芦头中人参皂苷的含量

Determination of Ginsenosides in Whole Ginseng, Fibrous Root and Root Refs by UPLC

doi:
10.3969/j.issn.0517-6611.2017.28.046
摘要:
[目的]建立适用于整参、须根、芦头中人参皂苷的超高效液相色谱(UPLC)检测方法,同时测定人参皂苷Rg1、Re、Rf、Rg2、Rb1、Rc、Rb2、Rd的含量.[方法]采用ACQUITY UPLC BEH C18色谱柱(1.7μm,2.1 mm×50 mm)分离,流动相为乙腈-0.1 mol/L磷酸溶液,梯度洗脱,检测波长为203 nm,柱温35℃,流速0.3 mL/min.[结果]人参皂苷Rg1、Re、Rf、Rg2、Rb1、RC、Rb2、Rd线性关系良好,回收率在87.3%~98.6%.[结论]该方法准确、重复性好,适用于整参、须根、芦头中人参皂苷的定量分析.
Abstract:
[Objective] The research aimed to establish a chromatographic separation method for the determination of ginsenosides in the whole ginseng, fibrous root and root refs.Ginsenosides Rg1,Re,Rf,Rg2,Rb1,Rc,Rb2 and Rd were determined.[Method]The separation was performed by a ACQUITY UPLC BEH C18 column (1.7 μm, 2.1 mm ×50 mm), the mobile phase was acetonitrile -0.1%phosphoric acid aqueous so-lution with gradient elution at a flow rate of 0.3 mL/min, the detection wavelength was set at 203 nm, and the column temperature was 35 ℃. [Result]The method showed a good linear relationship of ginsenosides Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rd.Recovery rate was from 87.3%to 98.6%.[Conclusion]This method is accurate and good reproducibility,and suitable for the determination of ginsenosides in the whole ginseng, fibrous root and root refs.
作者 赵琳琳    赵云平    魏静娜    马西兰    刘征辉
Author:
作者单位 天津海世达检测技术有限公司,天津300381;天津市农业质量标准与检测技术研究所,天津300381
刊 名: 安徽农业科学   ISTICPKU
Journal: Journal of Anhui Agricultural Sciences
年,卷(期) 2017, 45(28)
分类号 S567.5+1
关键词: 人参皂苷 知识脉络    超高效液相色谱法 知识脉络    整参 知识脉络    须根 知识脉络    芦头 知识脉络   
Keywords: Ginsenosides    UPLC    Whole ginseng    Fibrous root    Root refs   
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